Introduction: Oral squamous cell carcinoma is the sixth most common cancer worldwide and is one of the leading causes of death especially in developing countries. It is usually managed with the help of chemotherapeutic agents that are toxic not only to the tumor cells but also to normal cells in many ways. Whereas the traditional herbal medicines which are used from ancient days are generally known to be free of toxic effects and are effective. WHEATGRASS is the young grass of the wheat plant ‘Tritcumaestivum Linn’, family Poaceae (Gramineae). It contains a number of vitamins, mineral, amino acids and vital enzymes, these play an important role in the anticancer approach of this herbal product. It is also called as ‘green blood’ because of its high content of chlorophyll, which bears a structural similarity to haemoglobin and this result in high oxygen supply to all the body tissues.
Aim: To analyse the effect of the aqueous WHEATGRASS juice (extract) on Oral Squamous Cell Carcinoma (OSCC) cell line by MTT assay.
Materials and methods: ‘KB cell line’ (Mouth Epidermal Carcinoma Cells) was used for the present study. Aqueous extract (juice) of wheat grass was prepared in the institution. The aim of the study was to assess the effect of the aqueous WHEATGRASS on KB cell line(OSCC) by MTT assay.
There was no OSCC cells inhibition after incubating without wheat-grass extract.
15.6% OSCC cells were inhibited after incubating with wheatgrass extract (25 µg/ml).
23.4% OSCC cells were inhibited after incubating with wheatgrass extract (50 µg/ml).
24.6% OSCC cells were inhibited after incubating with wheatgrass extract (100 µg/ml).
29% OSCC cells were inhibited after incubating with wheatgrass extract (500 µg/ml).
41.4% OSCC cells were inhibited after incubating with wheatgrass extract (1000 µg/ml).









